Human T lymphocyte mitogenesis in response to the B oligomer of pertussis toxin is associated with an early elevation in cytosolic calcium concentrations

FEBS Letters

Carchman, Richard A.¹  Strnad, Colette F.¹ 

[1] Department of Pharmacology and Toxicology, Medical College of Virginia, Richmond, VA 23298-0001, USA

关键词: T lymphocyte;    Ca2+;    Pertussis toxin;    Mitogen;   

DOI  :  10.1016/0014-5793(87)81123-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pertussis toxin was found to serve as a mitogen in the human T lymphocyte, an effect which could be mimicked by its resolved binding component, the B oligomer. The mechanism of action of this component appeared to involve a rapid and sustained elevation of cytosolic calcium levels, as monitored by fura-2 fluorescence. The source of mobilized calcium was predominantly extracellular, suggesting that the binding of the B oligomer to the T cell plasma membrane in some way elicited calcium channel activation. Notably, the influx of calcium was not observed with cholera toxin, an AB toxin lacking mitogenic effects on the human T lymphocyte.

【 授权许可】

Unknown