Variability in electrophoretic mobility of Gi‐like proteins: effect of SDS

FEBS Letters

Schultz, Richard M.²  Kopf, Gregory S.¹  Jones, JoLynda² 

[1] Division of Reproductive Biology, University of Pennsylvania, Philadelphia, PA 19104, USA;Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA

关键词: G-protein;    ADP-ribosylation;    Pertussis toxin;    Electrophoresis;    SDS;   

DOI  :  10.1016/0014-5793(89)80172-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two forms of G_i-like protein are resolved in both somatic cells and mouse gametes when Sigma SDS (95% grade) is used during polyacrylamide gel electrophoresis, whereas only a single species is resolved when Bio-Rad SDS (electrophoresis grade) is used. These two G_i-like proteins are likely to reflect two distinct species, since (i) the two species resolved in the presence of Sigma SDS migrate with the same electrophoretic mobility upon re-electrophoresis in the presence of Sigma SDS and (ii) exchanging Sigma SDS for Bio-Rad SDS resolves a single species, whereas exchanging Bio-Rad SDS for Sigma SDS resolves two species.

【 授权许可】

Unknown