FEBS Letters | |
Variability in electrophoretic mobility of Gi‐like proteins: effect of SDS | |
Schultz, Richard M.2  Kopf, Gregory S.1  Jones, JoLynda2  | |
[1] Division of Reproductive Biology, University of Pennsylvania, Philadelphia, PA 19104, USA;Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA | |
关键词: G-protein; ADP-ribosylation; Pertussis toxin; Electrophoresis; SDS; | |
DOI : 10.1016/0014-5793(89)80172-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two forms of Gi-like protein are resolved in both somatic cells and mouse gametes when Sigma SDS (95% grade) is used during polyacrylamide gel electrophoresis, whereas only a single species is resolved when Bio-Rad SDS (electrophoresis grade) is used. These two Gi-like proteins are likely to reflect two distinct species, since (i) the two species resolved in the presence of Sigma SDS migrate with the same electrophoretic mobility upon re-electrophoresis in the presence of Sigma SDS and (ii) exchanging Sigma SDS for Bio-Rad SDS resolves a single species, whereas exchanging Bio-Rad SDS for Sigma SDS resolves two species.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020291594ZK.pdf | 311KB | download |