Isolated fungal cellulose terminal domains and a synthetic minimum analogue bind to cellulose

FEBS Letters

Johansson, Gunnar¹  Lindeberg, Gunnar²  Pettersson, Göran¹  Ståhlberg, Jerry¹  Engström, Åke² 

[1] Department of Biochemistry, University of Uppsala, Box 576 BMC S- 751 23 Uppsala, Sweden;Department of Immunology, University of Uppsala, Box 582 BMC, S- 751 23 Uppsala, Sweden

关键词: Cellulase;    Binding domain;    Synthetic domain;    Cellulose binding;    Fluorescence;    (Trichoderma reesei;    Sporotrichum pulverulentum);    Tr;    Trichoderma reesei;    Sp;    Sporotrichum pulverulentum;    Boc;    t-butyloxycarbonyl;    Bzl;    benzyl;    Br-Z;    2-bromobenzyloxycarbonyl;    Me-Bzl;    4-methylbenzyl;    Dnp;    2;    4-dinitrophenyl;    DMF;    dimethyl formamide;    DCM;    dichloromethane;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/0014-5793(89)80168-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cellulose-binding properties of the highly conserved terminal region which is common to several fungal cellulases were studied. Domains were prepared by proteolytic cleavage of Trichoderma reesei CBH1 and the corresponding enzyme from Sporotrichum pulverulentum, and a peptide corresponding to residues 462–497 (the C-terminal part) of Trichoderma CBH1 was synthesized. The three peptides showed similar binding behavior, whereas reduced and S-carboxymethylated T. reesei fragment was inactive. This region thus appears to serve as an independent functional domain in which the C-terminal part is responsible for the binding, which in turn requires an intact three-dimensional structure.

【 授权许可】

Unknown