Polyamines inhibit the ATP‐dependent proteolytic pathway in rabbit reticulocyte lysates

FEBS Letters

Wajnberg, Ewa F.¹  Fagan, Julie M.¹ 

[1] Department of Animal Sciences, Rutgers University, New Brunswick, NJ 08903, USA

关键词: Polyamine;    Protein degradation;    ATP;    (Reticulocyte);   

DOI  :  10.1016/0014-5793(89)80116-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Reticulocytes contain a soluble nonlysosomal proteolytic pathway that requires ATP and ubiquitin. Polyamines at physiological concentrations were found to inhibit rapidly the ATP-dependent proteolytic system in reticulocyte lysates; spermidine and putrescine inhibited this process by 26–72% and spermine by 71–96%. Spermine had little effect on the ATP-independent breakdown of oxidant-treated hemoglobin. By fractionating the ATP-dependent system, we show that polyamines inhibit the ATP-dependent degradation of ubiquitin-protein conjugates.

【 授权许可】

Unknown