Prediction of terminal protein and ribonuclease H domains in the gene P product of hepadnaviruses

FEBS Letters

Makhov, A.M.¹  Khudyakov, Yu.E.¹ 

[1] D.I. Ivanovsky Institute of Virology, Academy of Medical Sciences of the USSR, Gamaleya st. 16, Moscow 123098, USSR

关键词: Reverse transcriptase;    Ribonuclease H;    Terminal protein;    Domain structure;    Protein homology;   

DOI  :  10.1016/0014-5793(89)80110-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By means of comparative analysis of primary and secondary structures, and hydropathy plots of hepadnavirus P proteins new functional domains were revealed additionally to the polymerase domain which had been found earlier in these proteins. The C-terminal part of P proteins was revealed to be significantly similar to ribonuclease H of E. coli. The ribonuclease H functional domain is known to be an integral entity of retrovirus reverse transcriptase as a rule. Availability of this domain indicates once more the putative reverse transcriptase properties of the P products. The proteins of hepadnaviruses were compared to terminal proteins of picornaviruses, adenoviruses and bacteriophages. The data obtained suggested that a conservative N-terminal region of P proteins functions as protein primer for DNA synthesis in hepadnaviruses.

【 授权许可】

Unknown