Human immunodeficiency virus type 1 (HIV‐1) recombinant reverse transcriptase

FEBS Letters

Evans, David B.¹  Fan, Naisheng¹  Sharma, Satish K.¹ 

[1] Biochemistry, Upjohn Laboratories, Kalamazoo, MI 49002, USA

关键词: HIV-1;    Reverse transcriptase;    p66/p66 homodimer;    Ribonuclease H;    Protein asymmetry;    HPLC;    high pressure liquid chromatography;    HIV-1;    human immunodeficiency virus type 1;    RT;    reverse transcriptase;    RNase H;    ribonuclease H;    IMAC;    immobilized metal affinity chromatography;    SDS;    sodium dodecylsulfate;    DTT;    dithiothreitol;    IDA;    iminodiacetic acid;    TCA;    trichloroacetic acid;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(94)80303-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recombinant p66 form of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) can be obtained [(1991) Biotechnol. Appl. Biochem. 14, 69-81] from crude Escherichia coli extracts by immobilized metal affinity chromatography (IMAC). We have analyzed the p66 HIV-1 RT, isolated in the presence of 0.3 M imidazole, by gel permeation HPLC on Superose 12. The results show that it contains two major distinct p66 forms (24.1 min and 28.3 min peaks) which are distinguishable from the purified homodimeric (p66/p66) HIV-1 RT (22.2 min peak). Protein peak 1 (24.1 min) is converted to a 22.3 min peak upon storage for 20 h at 4°C. Under identical conditions, the isolated peak 2 (28.3 min) appeared as a conformationally heterogeneous mixture elaborated by peaks at 22.3 min and 25.9 min. The protein species thus obtained were active in the RNA-dependent DNA polymerase and RNase H activity assays and produced heterodimeric HIV-1 RT upon incubation with the HIV-1 protease. When the IMAC-purified, imidazole-free homodimeric (p66/p66) form of the enzyme was incubated with 0.3 M imidazole for 16 h at 4°C, protein peaks at 28.3 min (peak A) and 30.5 min (peak B) were isolated by gel permeation HPLC. While both of these p66-containing species were stable and displayed identical RNA-dependent DNA polymerase activities, the protein in peak B was only 50% active in RNase H function compared with the protein from peak A. These imidazole-mediated dissociation studies support the hypothesis of partial unfolding of one of the RNase H domains of the p66/p66 homodimer, suggesting that the p66 subunits are asymmetric in the native enzyme.

【 授权许可】

Unknown