Interaction between bacteriophage T4 coded gene 32 protein and poly(rA)

FEBS Letters

Watanabe, Fumiyuki¹ 

[1] Department of Biophysical Chemistry, Biocenter, University of Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland

关键词: Gene protein;    DNA-binding protein;    single-stranded;    Cooperativity;    DNA-protein interaction;    Thermodynamics;   

DOI  :  10.1016/0014-5793(89)80519-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cooperative binding of T₄ gene 32 protein with polynucleotides, of which the quantitative aspects in the literature have not satisfied the requirements of thermodynamics, is studied by adopting a modified formula of the lattice theory. A moderate value is found for the cooperativity parameter (q ∼ 200 at 0.2 M NaCl), which is weakly dependent on salt concentration. The cation effect on the binding suggests that the shielding of negative charges of the protein or a loose cation bridge between the bound protein molecules plays a role in the cooperative binding process.

【 授权许可】

Unknown