The algorithm of estimation of the Kmvalues for primers in DNA synthesis catalyzed by human DNA polymerase α

FEBS Letters

Nevinsky, Georgy A.¹  Kolocheva, Tat'yana I.¹  Maksakova, Galiya A.¹  Zakharova, Ol'ga D.¹ 

[1] Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the Russian Academy of Sciences, Lavrentyeva Ave, 8, Novosibirsk 630090, Russian Federation

关键词: DNA polymerase a;    Human placenta;    Kinetics;    Thermodynamics;    HDPA;    human DNA polymerase α;    KF;    Klenow fragment of E. coli DNA polymerase 1;    ODN;    oligodeoxynucleotide;    ss;    single-stranded;    ds;    double-stranded;    respectively;   

DOI  :  10.1016/S0014-5793(96)01298-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA synthesis with various deoxyribo homo- and heterooligoprimers in the presence of complementary templates was investigated. The lengthening of d(pN) _n primers (n =1–10) by a unit resulted in an increase of the primer affinity and a maximal rate of polymerization. The coefficient of the affinity enhancement of primers due to formation of one hydrogen bond between primer and template was found to be 1.35. The dependence of the primer affinity and polymerization rate on template-primer structure in solution was analyzed and the objective laws of the changes of the KM and V _max values were revealed.

【 授权许可】

Unknown