DNA replication specificity of TYLCV geminivirus is mediated by the amino‐terminal 116 amino acids of the Rep protein

FEBS Letters

Jupin, Isabelle¹  Benz, Bianca¹  Hericourt, François¹  Gronenborn, Bruno¹ 

[1] Institut des Sciences Végétales, CNRS, Avenue de la Terrasse, 91198 Gif sur Yvette Cedex, France

关键词: Geminivirus;    TYLCV;    DNA replication;    Rolling circle;    Specificity of origin recognition;    aa;    amino acid;    nt;    nucleotide;    ss;    single-stranded;    IR;    intergenic region;    ORF;    open reading frame;    BGMV;    bean golden mosaic virus;    SqLCV;    squash leaf curl virus;    TGMV;    tomato golden mosaic virus;    TYLCV;    tomato yellow leaf curl virus;   

DOI  :  10.1016/0014-5793(95)00221-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Geminiviruses are plant DNA viruses replicating by a rolling circle mechanism. We have investigated the specificity of replication origin recognition of two different isolates of tomato yellow leaf curl virus (TYLCV). Here, we show that TYLCV-Sardinian and -Israeli replication proteins display a high degree of specificity for their respective origins. The DNA sequences recognized are located on the left part of the intergenic region whereas the amino-terminal 116 amino acids of the Rep protein determine the specificity of origin recognition.

【 授权许可】

Unknown