Agrobacterium tumefaciens supports DNA replication of diverse geminivirus types

FEBS Letters

Rezaian, M.Ali²  Selth, Luke A.²  Randles, John W.¹ 

[1] Department of Applied and Molecular Ecology, Adelaide University, Adelaide, SA, Australia;CSIRO Plant Industry (Horticulture Unit), PO Box 350, Glen Osmond, SA, Australia

关键词: Geminivirus;    Begomovirus;    Replication;    β-Glucuronidase;    Agrobacterium tumefaciens;    Escherichia coli;    ACMV;    African cassava mosaic virus;    GUS;    β-glucuronidase;    TLCV;    tomato leaf curl virus;    TYLCV;    tomato yellow leaf curl virus;   

DOI  :  10.1016/S0014-5793(02)02539-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously shown that the soil-borne plant pathogen Agrobacterium tumefaciens supports the replication of tomato leaf curl geminivirus (Australian isolate) (TLCV) DNA. However, the reproducibility of this observation with other geminiviruses has been questioned. Here, we show that replicative DNA forms of three other geminiviruses also accumulate at varying levels in Agrobacterium. Geminiviral DNA constructs that lacked the ability to replicate in Agrobacterium were rendered replication-competent by changing their configuration so that two copies of the viral ori were present. Furthermore, we report that low-level replication of TLCV DNA can occur in Escherichia coli containing a dimeric TLCV construct in a high copy number plasmid. These findings were reinforced by expression studies using β-glucuronidase which revealed that all six TLCV promoters are active in Agrobacterium, and two are functional in E. coli.

【 授权许可】

Unknown