A 10 kDa polypeptide associated with the oxygen‐evolving complex of photosystem II has a putative C‐terminal non‐cleavable thylakoid transfer domain

FEBS Letters

Webber, Andrew N.²  Packman, Leonard C.¹  Gray, John C.² 

[1] Biochemistry, University of Cambridge, Cambridge CB2 3EA, England;Departments of Botany, University of Cambridge, Cambridge CB2 3EA, England

关键词: Chloroplast;    Photosystem II;    Oxygen-evolving complex;    Presequence;    Thylakoid transfer domain;    (Wheat);    PVDF;    polyvinylidene difluoride;    PTH;    phenylthiohydantoin;   

DOI  :  10.1016/0014-5793(89)80517-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The N-terminal amino acid sequence of the 10 kDa polypeptide associated with the oxygen-evolving complex of wheat photosystem II has been determined and shown to be homologous to the amino acid sequence of the product of the ST-LS1 gene from potato. The N-terminal sequence of the mature protein indicates that the polypeptide is synthesized with a 39 amino acid N-terminal presequence which is similar to chloroplast import sequences but which lacks a hydrophobic domain for transfer of the protein across the thylakoid membrane. The mature polypeptide has a C-terminal hydrophobic region which shows homology to the hydrophobic thylakoid transfer domain of other lumenal proteins and this hydrophobic region of the 10 kDa polypeptide is suggested to facilitate transfer of the protein across the thylakoid membrane.

【 授权许可】

Unknown