Effect of redox potential on rat liver F1‐ATPase

FEBS Letters

López-Moratalla, N.¹  López-Zabalza, M.J.¹  Flores, F.¹  Santiago, E.¹ 

[1] Department of Biochemistry, University of Navarra, Pamplona, Spain

关键词: Mitochondrial ATPase;    F1-ATPase;    Redox titration;   

DOI  :  10.1016/0014-5793(89)80487-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Redox titration of F₁-ATPase from rat liver mitochondria referred to the modification of the hydrolytic activity on Mg-ATP has resulted in a three-step pattern, with three distinct jumps of activity separated by clear plateaus. The measured potentials ranged from −400 mV to +400 mV and were obtained by the addition of dithionite and ferricyanide. Electron exchange was facilitated with a mixture of different redox mediators. At pH 7.4 the midpoint potentials were +210 mV, +40 mV and −230 mV. These three midpoint potentials were displaced towards more negative values by 2,4-dinitrophenol or by an increase of the pH of the medium. The titration curves were described by n=2 Nernst equations.

【 授权许可】

Unknown