Increased muscarinic receptor binding in heart membranes by an inhibitor of protein kinase C

FEBS Letters

Wrenn, Robert W.¹  Creazzo, Tony L.¹ 

[1] Medical College of Georgia, Department of Anatomy, Augusta, GA 30912-2000, USA

关键词: Muscarinic receptor;    Phosphorylation;    Acetylcholine;    (Heart);   

DOI  :  10.1016/0014-5793(88)81010-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [³H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg²⁺ reduced MAChR number by 50%. Incubation with polymyxin B, an inhibitor of protein kinase C, blocked the efrect of ATP/Mg²⁺ and increased MAChR number by 74%.

【 授权许可】

Unknown