Ionic currents in avian granulosa cells

FEBS Letters

Schwartz, Jean-Louis²  Whitfield, James F.²  Asem, Elikplimi K.¹  Mealing, Geoffrey A.R.²  Tsang, Benjamin K.¹ 

[1] Reproductive Biology Unit, Department of Obstetrics and Gynecology, University of Ottawa, Ottawa Civic Hospital, Ottawa, Ontario K1Y 4E9, Canada;Cellular Oncology Group, Division of Biological Sciences, National Research Council of Canada, Ottawa, Ontario K1A 0R6 Canada

关键词: K+ current;    Ca2+ current;    Whole-cell patch-clamp;    (Granulosa cell);   

DOI  :  10.1016/0014-5793(88)81053-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transmembrane ionic currents have been recorded in single granulosa cells from the laying hen using the whole-cell patch-clamp technique. Under voltage-clamp conditions, depolarizing voltage steps evoked currents composed of a fast inactivating inward component and a delayed outward component. The former was activated at voltages more positive than −50 mV and was fully inactivated within 500 ms. It was blocked by D600 (methoxyverapamil) and by cobalt, suggesting that it is a calcium current. The latter displayed inward rectification and did not inactivate during long duration pulses. It was blocked by tetraethylammonium indicating that it is a potassium current. This is the first evidence of the existence of potassium and calcium transmembrane currents in granulosa cells.

【 授权许可】

Unknown