‘Perforated patch recording’ allows long‐term monitoring of metabolite‐induced electrical activity and voltage‐dependent Ca2+ currents in pancreatic islet B cells

FEBS Letters

Misler, Stanley¹  Falke, Lee C.¹  Gillis, Kevin D.¹  Pressel, David M.¹ 

[1] Departments of Medicine (Jewish Hospital), Cell Biology/Physiology and Electrical Engineering (Program in Biomedical Engineering), Washington University, St. Louis, MO 63110, USA

关键词: Patch recording;    permeabilized;    Nystatin;    Electrical activity;    Ca2+ current;    (Pancreatic B cell);   

DOI  :  10.1016/0014-5793(89)81448-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the application of ‘perforated patch recording’ using the pore-forming antibiotic nystatin, to monitor the electrical activity and underlying ionic currents of rat and human pancreatic islet B cells. We demonstrate that glucose-induced electrical activity is seen even in single B cells during current-clamp recordings lasting hours ‘L-type’ Ca²⁺-channel currents can also be monitored over this period of time. This technique may prove useful in examining hormone and neurotransmitter modulation of electrical activity in B cells, while minimizing the effects of cytoplasmic ‘wash-out’.

【 授权许可】

Unknown