Phorbol ester stimulates the synthesis of sphingomyelin in NIH 3T3 cells A diminished response in cells transformed with human A‐raf carrying retrovirus

FEBS Letters

Rapp, Ulf R.²  Anderson, Wayne B.¹  Kiss, Zoltan¹ 

[1] Division of Cancer Biology and Diagnosis, National Cancer Institute, NIH, Bethesda, MD 20892, USA;Laboratory of Viral Carcinogenesis, National Cancer Institute, Frederick Cancer Research Facility, Frederick, MD 21701-1013, USA

关键词: Phorbol ester;    Sphingomyelin synthesis;    CerPCho;    sphingomyelin;    PtdCho;    phosphatidylcholine;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    PKC;    protein kinase C;    EGF;    epidermal growth factor;   

DOI  :  10.1016/0014-5793(88)80372-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) stimulated the synthesis of sphingomyelin (CerPCho) from a [¹⁴C]choline-labelled phosphatidylcholine (PtdCho) pool in NIH 3T3 cells. Maximal stimulation (68%) of CerP-Cho synthesis, accompanied by an increase (38%) in its cellular content, required only 2 nM TPA. Higher concentrations of TPA (2–100 nM) had progressively less effect on CerPCho synthesis which correlated with increased hydrolysis of precursor PtdCho. In cells transformed with human or mouse A-raf carrying retroviruses TPA-stimulated PtdCho hydrolysis, but not CerPCho synthesis, suggesting independent regulation of these processes by the TPA-stimulated signal transduction system.

【 授权许可】

Unknown