Chicken liver basic fatty acid‐binding protein (pI = 9.0) Purification, crystallization and preliminary X‐ray data

FEBS Letters

Zanotti, Giuseppe¹  Monaco, Hugo L.²  Mammi, Mario¹  Scapin, Giovanna¹  Spadon, Paola¹  Pengo, Licia¹ 

[1] Biopolymer Research Center, Department of Organic Chemistry, University of Padova, 35100 Padova, Italy;Department of Genetics, University of Pavia, 27100 Pavia, Italy

关键词: Fatty acid-binding protein;    Crystal growth;    X-ray diffraction;    (Chicken liver);   

DOI  :  10.1016/0014-5793(88)80367-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chicken liver basic fatty acid-binding protein (pI = 9.0) has been purified with a high yield by a modification of a method originally applied to rat liver. The final product is highly homogeneous and can be used to grow crystals that belong to two different space groups. The crystals are either tetragonal, space group P4₂2₁2 with a = b = 60.2 Å and c = 138.1 Å or orthorhombic, space group P2₁2₁2₁ with a = 60.7 Å, b = 40.1 Å and c = 66.7 Å. The second form appears to be more suitable for X-ray diffraction studies, it diffracts to at least 2.8 Å resolution and it is believed to contain one protein molecule in the crystallographic asymmetric unit.

【 授权许可】

Unknown