Different tachykinin receptor subtypes are coupled to the phosphoinositide or cyclic AMP signal transduction pathways in rat submandibular cells

FEBS Letters

Sliwinski-Lis, E.¹  Fleming, N.¹  Laniyonu, A.¹ 

[1] Department of Oral Biology, University of Manitoba, 780 Bannatyne Avenue, Winnipeg, Manitoba R3EOW2, Canada

关键词: Substance P;    Tachykinin receptor;    Phospholipase C;    Adenylate cyclase;    G protein;    Pertussis toxin;    IP3;    inositol 1;    4;    5-trisphosphate;    PI;    phosphoinositide;    PLC;    phospholipase C;    DG;    diacylglycerol;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    SP;    substance P;    SPME;    substance P methyl ester;    IPR;    isoproterenol;    cAMP;    cyclic adenosine 3′;    5′-monophosphate;    PT;    pertussis toxin;    NKA;    neurokinin A;    NKB;    neurokinin B;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(88)80365-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tachykinins of different classes (NK1, NK2, NK3) caused the concentration-dependent synthesis of IP₃ in rat submandibular acinar cells with the potency rank order of NK1 < NK2 > NK3. Enhancement of IP₃ was not affected by pertussis toxin treatment. The reverse rank order was found in the tachykinin inhibition of isoproterenol-induced cAMP synthesis and this inhibition was abolished by pertussis toxin, an inactivator of the adenylate cyclase G_i regulatory protein. It is suggested that different tachykinin receptor subtypes are preferentially coupled to phospholipase C or adenylate cyclase by separate G regulatory proteins in rat submandibular acinar cells.

【 授权许可】

Unknown