α‐Bungarotoxin interacts with the rat brain tachykinin receptors

FEBS Letters

Tsetlin, V.I.¹  Lazakovich, E.M.¹  Utkin, Yu.N.¹  Kasheverov, I.E.¹ 

[1] Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow, USSR

关键词: α-Bungarotoxin;    Substance P;    Tachykinin receptor;    (Rat brain membrane);    AChR;    acetylcholine receptor;    αBgt;    α-bungarotoxin;    BH-EL and BH-SP;    eledoisin and substance P modified with Bolton-Hunter reagent;    CHAPS;    (3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate;    EL;    eledoisin;    SP;    substance P;    TChR;    tachykinin receptor;   

DOI  :  10.1016/0014-5793(89)81071-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Bungarotoxin (αBgt) was shown to inhibit the binding of the ¹²⁵I-labeled substance P (SP) and eledoisin (EL) to the rat brain membranes with K ₁ values of 8.0±5.0 × 10⁻⁸ and 1.1±0.5 × 10⁻⁶ M, respectively. Lower inhibitory activity was manifested by several other postsynaptically acting snake venom neurotoxins. The αBgt inhibition of SP binding with a K ₁ value of 8.5±5.5 × 10⁻⁸ M to solubilized preparations of the rat brain membranes was demonstrated. The capacity to displace SP was found for d-tubocurarine and phencyclidine, although at concentrations considerably higher than those affecting the nicotinic acetylcholine receptors (AChRs). The results obtained suggest that some of the αBgt-binding polypeptides, distinct from neuronal AChRs, may be functionally associated with the tachykinin receptors (TchR).

【 授权许可】

Unknown