Occurrence and spacing of ribosome recognition sites in mRNAs of chloroplasts from higher plants

FEBS Letters

Ruf, Michael¹  Kössel, Hans¹ 

[1] Institut für Biologie III der Universität Freiburg, Schänzle-Str. 1, D- 7800 Freiburg, FRG

关键词: Chloroplast;    Translational initiation;    Ribosome recognition site;   

DOI  :  10.1016/0014-5793(88)80336-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A computer-aided search for potential ribosome recognition sequences of mRNAs from tobacco chloroplasts shows that more than 90% of mRNA species contain sequences upstream of the respective initiator codons, which allow base pairing with 3′-terminal sequences of small subunit rRNA. This complementarity in several cases involves 16 S rRNA sequences between the canonical CCUCC sequence and the 3′-terminal stem/loop structure. The distances between potential ribosome recognition sequences and initiator codons can be up to 25 nucleotides which is much greater when compared to the spacing of 7±2 nucleotides observed for the classical Shine-Dalgarno sequences in bacterial mRNAs.

【 授权许可】

Unknown