FEBS Letters | |
Ribosome‐messenger recognition in the absence of the Shine‐Dalgarno interactions | |
Tzareva, N.V.1  Makhno, V.I.2  Boni, I.V.1  | |
[1] M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871 Moscow, Russian Federation;Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188350 Gatchina, Russian Federation | |
关键词: Translational initiation; RNA-protein interaction; Plant viral RNA leader; Protein S1; Toeprinting; Footprinting; Omega (Ω); 5'-untranslated sequence of tobacco mosaic virus (TMV) RNA; A1MV RNA 4; the subgenomic mRNA for alfalfa mosaic virus coat protein; GUS; the sequence coding for β-glucuronidase of Escherichia coli; RT; reverse transcription; AMV RTase; reverse transcriptase from avian myoblastosis virus; | |
DOI : 10.1016/0014-5793(94)80271-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgamo (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in E. coli: the plant viral messenger A1MV RNA 4 and two chimaeric mRNAs coding for β-glucuronidase (GUS) and bearing the 5'-untranslated sequence of TMV RNA Ω or the Ω-derived sequence (CAA)n as 5'-leaders. Ribosomal protein Sl and IF3 have been found to be indispensable for translational initiation. Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes.
【 授权许可】
Unknown
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