Ribosome‐messenger recognition in the absence of the Shine‐Dalgarno interactions

FEBS Letters

Tzareva, N.V.¹  Makhno, V.I.²  Boni, I.V.¹ 

[1] M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871 Moscow, Russian Federation;Petersburg Nuclear Physics Institute, Russian Academy of Sciences, 188350 Gatchina, Russian Federation

关键词: Translational initiation;    RNA-protein interaction;    Plant viral RNA leader;    Protein S1;    Toeprinting;    Footprinting;    Omega (Ω);    5'-untranslated sequence of tobacco mosaic virus (TMV) RNA;    A1MV RNA 4;    the subgenomic mRNA for alfalfa mosaic virus coat protein;    GUS;    the sequence coding for β-glucuronidase of Escherichia coli;    RT;    reverse transcription;    AMV RTase;    reverse transcriptase from avian myoblastosis virus;   

DOI  :  10.1016/0014-5793(94)80271-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In an attempt to understand how Escherichia coli ribosomes recognize the initiator codon on mRNAs lacking the Shine-Dalgamo (SD) sequence, we have studied 30S initiation complex formation in extension inhibition (toeprinting) experiments using (-SD)mRNAs which are known to be reliably translated in E. coli: the plant viral messenger A1MV RNA 4 and two chimaeric mRNAs coding for β-glucuronidase (GUS) and bearing the 5'-untranslated sequence of TMV RNA Ω or the Ω-derived sequence (CAA)_n as 5'-leaders. Ribosomal protein Sl and IF3 have been found to be indispensable for translational initiation. Protein S1 appears to be a key recognition element. S1 binds to sequences within the leaders of (-SD)mRNAs thus providing their affinity to E. coli ribosomes.

【 授权许可】

Unknown