Low‐cytopathic infectious clone of human immunodeficiency virus type I (HIV‐I)

FEBS Letters

Ma, Xiaoyue¹  Volsky, David J.¹  Sakai, Koji¹ 

[1] Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center and College of Physicians & Surgeons, Columbia University, 432 West 58th Street, New York, NY 10019, USA

关键词: HIV-I variant;    Cytopathogenicity;    Restriction map;    Biological activity;    HIV;    human immunodeficiency virus;    AIDS;    acquired immunodeficiency syndrome;    IF;    indirect immunofluorescence staining;    RT;    reverse transcriptase;   

DOI  :  10.1016/0014-5793(88)80491-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Single genotypic variants of HIV-I, contained in a parental cytopathic HIV-I isolate, were isolated by molecular cloning and propagated in susceptible cells. Two such HIV-I clones, designated N1T-E and N1T-A, exhibited similar restriction endonuclease maps but strikingly different biological activities. Infection of T lymphocytes or monocytes by clone N1T-E was characterized by slow kinetics and lack of significant cytopathic effects, but high reverse transcriptase activity levels in culture supernatants of chronically-infected cells. Clone N1T-A, like the parental HIV-I isolate, exhibited fast kinetics of infection in T cells and monocytes and strong cytopathicity in these cells. Full characterization of the low-cytopathic virus in comparison to the structurally similar cytopathic clone may facilitate the elucidation of the molecular basis of HIV cytopathogenicity.

【 授权许可】

Unknown