Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits

PeerJ

article

Amudha Deepalakshmi Maharajan¹  Hilde Hansen¹  Miriam Khider²  Nils Peder Willassen¹ 

[1] Norwegian Structural Biology Center and The Department of Chemistry, Faculty of Science and Technology, UiT-The Arctic University of Norway;Department of Public Health and Nursing, Faculty of Medicine and Health Sciences, NTNU-Norwegian University of Science and Technology;Centre for Bioinformatics, Department of Chemistry, Faculty of Science and Technology, UiT-The Arctic University of Norway

关键词: Quorum sensing;    AHL;    Aliivibrio wodanis;    Winter ulcer;    Atlantic salmon;    Motility;    Protease;    Cytopathogenicity;    Siderophores;    CHSE;   

DOI  :  10.7717/peerj.11980

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

BackgroundQuorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ulcer of a reared Atlantic salmon, produces the autoinducer N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL) and encodes the QS systems AinS/R and LuxS/PQ, and the master regulator LitR. However, the role of QS in this bacterium has not been investigated yet.ResultsIn the present work we show that 3OHC10-HSL production is cell density and temperature-dependent in A. wodanis 06/09/139 with the highest production occurring at a low temperature (6 °C). Gene inactivation demonstrates that AinS is responsible for 3OHC10-HSL production and positively regulated by LitR. Inactivation of ainS and litR further show that QS is involved in the regulation of growth, motility, hemolysis, protease activity and siderophore production. Of these QS regulated activities, only the protease activity was found to be independent of LitR. Lastly, supernatants harvested from the wild type and the ΔainS and ΔlitR mutants at high cell densities show that inactivation of QS leads to a decreased cytopathogenic effect (CPE) in a cell culture assay, and strongest attenuation of the CPE was observed with supernatants harvested from the ΔlitR mutant.ConclusionA. wodanis 06/09/139 use QS to regulate a number of activities that may prove important for host colonization or interactions. The temperature of 6 °C that is in the temperature range at which winter ulcer occurs, plays a role in AHL production and development of CPE on a Chinook Salmon Embryo (CHSE) cell line.

【 授权许可】

CC BY