Molecular cloning of unintegrated closed circular DNA of porcine retrovirus

FEBS Letters

Shimizu, Nobuaki¹  Shimotohno, Kunitada¹  Suzuka, Iwao²  Hoshino, Hiroo¹  Kodama, Michi²  Sekiguchi, Kiichi² 

[1] National Cancer Center Research Institute, Chuoh-ku, Tokyo 104, Japan;National Institute of Animal Health, Tsukuba Science City, Ibaraki 305 Japan

关键词: closed circular DNA;    Cloning;    viral DNA;    Restriction map;    kb;    kilobase pairs;    cDNA;    DNA complementary to viral mRNA;    ccDNA;    closed circular DNA;    PRetV;    porcine retrovirus Tsukuba-1;    SKL;    swine kidney cell line;    LTR;    long terminal repeat;    EtBr;    ethidium bromide;   

DOI  :  10.1016/0014-5793(86)80432-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Viral DNA of unintegrated closed circular form was isolated from a swine kidney cell line (SKL) which was infected with a porcine retrovirus Tsukuba-1 (PRetV) produced from a swine malignant lymphoma-derived cell line, Shimozuma-1 and cloned using a λ phage vector; Charon 21 A. One of ten independent clones contained the 8.3 kb DNA fragment as an insert, which was thought to be a full length of viral DNA molecule carrying a long terminal repeat (LTR) sequence. We have analyzed this insert by mapping the recognition sites of some restriction endonucleases by Southern blot hybridization with appropriate probes.

【 授权许可】

Unknown