Phosphatidylinositol kinase activity of a plasma membrane‐associated calcium‐activated protein kinase from pea

FEBS Letters

Blowers, D.P.¹  Trewavas, A.J.¹ 

[1] Department of Botany, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JH, Scotland

关键词: Lipid kinase;    Phosphatidylinositol phosphorylation;    Phosphatidylinositol phosphate;    (Pisum sativum);    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol monophosphate;    PIP2;    phosphatidylinositol bisphosphate;   

DOI  :  10.1016/0014-5793(88)80231-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A purified protein kinase preparation from pea is shown to contain phosphatidylinositol kinase activity. The preparation, produced by membrane solubilisation and ATP-agarose affinity chromatography, contains proteins of 18 kDa (ppl8, a calcium activated autophosphorylating protein-serine kinase), 67 kDa and 48 kDa and exhibits the phosphorylation of an endogenous lipid when supplied with [γ-³²P]ATP. This product is tentatively identified as lysophosphatidylinositol phosphate. When supplied with [γ-³²P]ATP and exogenous phosphatidylinositol, the production of phosphatidylinositol phosphate is observed.

【 授权许可】

Unknown