Extraction of electron acceptor A1 from pea photosystem I

FEBS Letters

Hubbard, J.A.M.¹  Evans, M.C.W.¹  Nugent, J.H.A.¹  Mansfield, R.W.¹ 

[1] Department of Botany and Microbiology, University College London, Gower Street, London WC1E 6BT, England

关键词: Photosystem I;    Electron acceptor;    Ether extraction;    (Pisum sativum);    Chl;    chlorophyll;    ESR;    electron spin resonance;    PS;    photosystem;   

DOI  :  10.1016/0014-5793(87)80878-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosystem I particles were washed twice in either 0, 50 or 100% water-saturated diethyl ether. It was found that the characteristic electron spin resonance signal associated with electron acceptor A₁ was progressively lost with increasing percentage saturation of the ether. Light-induced electron flow to the terminal iron-sulphur acceptors was inhibited although these acceptors were still present and could be chemically reduced. The kinetics of optical measurements of P700⁺ re-reduction at 820 nm following ether washing were consistent with removal of electron acceptor A₁.

【 授权许可】

Unknown