Evidence for lipid kinase activities in spinach chloroplast envelope membranes

FEBS Letters

Müller, Marc-Olivier¹  Bovet, Lucien¹  Siegenthaler, Paul-André¹ 

[1] Laboratoire de Physiologie Végétale, Université de Neuchâtel, Rue Emile Argand 13, CH-2007 Neuchâtel, Switzerland

关键词: Acyl lipid;    Chloroplast envelope membrane;    Lipid kinase;    (Lyso) phosphatidic acid;    (Lyso) l-α-phosphatidyl-inositol 4-monophosphate;    Spinacia oleracea L.;    DAG;    diacylglycerol;    DGDG;    digalactosyldiacylglycerol;    GP;    l-glycerol-3-phosphate;    GPC;    l-α-glycerophosphorylcholine;    GPG;    l-α-glycerophosphorylglycerol;    GPIP;    l-α-glycerophospho-d-myo-inositol-4-monophosphate;    GPIP2;    l-α-glycerophospho-d-myo-inositol-4;    5-bis-phosphate;    HPTLC;    high performance TLC;    LMW;    low molecular weight;    LPA;    l-α-lysophosphatidic acid;    LPIP;    l-α-lysophosphatidyl-inositol 4-monophosphate;    MGDG;    monogalactosyldiacylglycerol;    Mops;    3-(N-morpholino)propane sulfonic acid;    PA;    l-α-phosphatidic acid;    PC;    phosphatidylcholine;    PG;    phosphatidylglycerol;    PI;    l-α-phosphatidyl-inositol;    PIP;    l-α-phosphatidyl-inositol 4-monophosphate;    PIP2;    l-α-phosphatidyl-inositol 4;    5-diphosphate;    PS;    l-α-phosphatidyl-l-serine;    SQDG;    sulfoquinovosyldiacylglycerol;    TLC;    thin layer chromatography;    Tris;    Tris(hydroxymethyl)aminomethane;   

DOI  :  10.1016/S0014-5793(97)01168-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three spinach chloroplast envelope membrane preparations (i.e. whole, outer and inner membranes) were incubated in the presence of [γ-³²P]ATP. After lipid extraction and separation by TLC, four main phosphorylated lipids were detected by autoradiography in whole envelope preparations. These phospholipids were identified by comparing their Rf with that of lipid markers and by a deacylation procedure. They were found to be phosphatidic acid (PA) and lyso-PA, l-α-phosphatidyl-inositol 4-monophosphate (PIP) and lyso-PIP. These lipids were not equally distributed in the outer and inner envelope membranes. Chloroplast envelope membranes were verified not to be contaminated by plasma membranes. It is concluded that lipid kinase activities are associated with spinach chloroplast envelope membranes.

【 授权许可】

Unknown