Evidence for a single glycan moiety in rabbit serum transferrin and location of the glycan within the polypeptide chain

FEBS Letters

Patel, Kokila J.¹  Evans, Robert W.¹  Aitken, Alastair² 

[1] Division of Biochemistry, UMDS, Guy's Hospital, London SE1 9R T England;Laboratory of Protein Structure, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: Transferrin;    Glycan;    Amino acid sequence;    Cyanogen bromide fragment;    (Rabbit);   

DOI  :  10.1016/0014-5793(88)80221-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequential removal of N-acetylneuraminic acid from rabbit serum transferrin has been followed by urea-polyacryl-amide gel electrophoresis. The electrophoretic pattern is consistent with the presence of a single biantennary glycan chain. From the amino acid sequence of the carbohydrate-containing cyanogen bromide fragment we have shown that the glycan is attached to an asparaginyl side chain at a position equivalent to residue 491 in the sequence of human serum transferrin.

【 授权许可】

Unknown