Role of N‐glycans and SDF‐1α on the coassociation of CD4 with CXCR4 at the plasma membrane of monocytic cells and blood lymphocytes

FEBS Letters

Mbemba, Elisabeth¹  Gattegno, Liliane¹  Saffar, Line¹ 

[1] Laboratoire de Biologie Cellulaire, EA 3510, Biothérapies, Bénéfices et Risques, Unité de Formation et de Recherche-Santé Médecine et Biologie Humaine, Université Paris XIII, 74 rue Marcel Cachin, 93017 Bobigny, France

关键词: CD4;    CXCR4;    Glycan;    SDF-1α;    PBL;    peripheral blood lymphocytes;    SDF-1α;    stromal cell derived factor-1α;    RANTES;    regulated on activation normal T cells expressed and secreted;    MIP;    macrophage inflammatory protein;    GAGs;    glycosaminoglycans;    gp;    glycoprotein;    12G5;    anti-CXCR4 mAb 12G5;    G19;    anti-CXCR4 polyclonal antibodies G19;   

DOI  :  10.1016/S0014-5793(02)02366-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

CXCR4 is a coreceptor, along with CD4, for human immunodeficiency virus type 1 (HIV-1). Trimolecular complexes between HIV-1 glycoprotein (gp)120, CD4 and CXCR4 constitute a prerequisite for HIV entry. We studied whether CD4 is associated with CXCR4 on CD4+ CXCR4+ cells. Using the conformation-dependent anti-CXCR4 mAb 12G5, CD4 was coimmunoprecipitated with CXCR4 from the membrane of U937 cells which support HIV-1_LAI efficient infection, and from that of peripheral blood lymphocytes (PBL). CD4 association with CXCR4 increased upon PBL coculture for 5 days with autologous monocytes, decreased upon treatment of the cells or the CD4-CXCR4 complex with either N-glycanase or stromal cell derived factor-1α (SDF-1α) and was abolished by incubation of the cells with both, N-glycanase and SDF-1α. This indicates that glycans are partly involved in CD4 association with CXCR4 and may partly explain the inhibitory effect of SDF-1α on HIV infection.

【 授权许可】

Unknown