The G protein β3 subunit 825T allele is a genetic marker for enhanced T cell response

FEBS Letters

Grosse-Wilde, Hans²  Lindemann, Monika²  Kreuzfelder, Ernst²  Virchow, Sebastian³  Müller, Norbert¹  Ramann, Frank³  Siffert, Winfried³  Barsegian, Vahé² 

[1] Institut für Transfusionsmedizin, Universitätsklinikum, 45122 Essen, Germany;Institut für Immunologie, Universitätsklinikum, 45122 Essen, Germany;Institut für Pharmakologie, Universitätsklinikum, 45122 Essen, Germany

关键词: Lymphocyte;    Proliferation;    Chemotaxis;    CD4;    G protein;    Genotype;    cpm;    counts per minute;    Gi protein;    inhibitory G protein;    GNB3;    G protein β3 subunit;    HBV;    hepatitis B virus;    HPF;    high power field;    HSV;    herpes simplex virus;    IL-2;    interleukin-2;    LTT;    lymphocyte transformation test;    MFI;    mean fluorescence intensity;    PBL;    peripheral blood lymphocyte;    PBMC;    peripheral blood mononuclear cell;    PHA;    phytohemagglutinin;    PPD;    tuberculin;    PTX;    pertussis toxin;    SDF-1α;    stromal cell-derived factor 1α;    S.E.M.;    standard error of the mean;   

DOI  :  10.1016/S0014-5793(01)02339-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The G protein β3 subunit (GNB3) 825T allele is predictive of enhanced Gi protein activation. Studying the influence of C825T allele status on cellular in vitro immune responses towards recall antigens and interleukin-2 stimulation we observed a 2–4-fold, significantly increased proliferation in homozygous 825T (TT) vs. C825 allele (CC) carriers. Furthermore, lymphocyte chemotaxis and CD4⁺ T cell counts of individuals with TT+TC genotypes were significantly enhanced compared to the CC genotype. In summary, it appears that C825T allele status is highly predictive of immunocompetence and could be a candidate gene in disorders associated with inadequate immune response.

【 授权许可】

Unknown