Differential down‐regulation of protein kinase C subspecies in KM3 cells

FEBS Letters

Ase, Katsuhiko¹  Kishimoto, Akira¹  Berry, Nicola¹  Kikkawa, Ushio¹  Nishizuka, Yasutomi¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: Protein kinase C;    Enzyme subspecies;    Down-regulation;    PKC;    protein kinase C;    FPLC;    fast protein liquid chromatography;    TPA;    12-O-tetradecanoylphorbol 13-acetate;   

DOI  :  10.1016/0014-5793(88)80064-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The down-regulation of protein kinase C (PKC) subspecies in KM3 cells (a pre-B, pre-T cell line) has been examined. The PKC from KM3 cells was resolved into two subspecies, type II (mainly βII) and type III (α), upon hydroxyapatite column chromatography. Biochemical and immunocytochemical analysis revealed that, when these cells were treated with 12-O-tetradecanoylphorbol 13-acetate (TPA), the time course of down-regulation of the PKC subspecies was different; type II PKC was translocated and depleted from the cell more quickly than type III enzyme. The results suggest that each PKC subspecies plays a different role in the cellular response to TPA and probably to other external stimuli.

【 授权许可】

Unknown