FEBS Letters | |
Protein kinase C isoforms in murine erythroleukemia cells and their involvement in the differentiation process | |
Pontremoli, S.1  Sparatore, B.1  Passalacqua, M.1  Melloni, E.1  Patrone, M.1  Pessino, A.1  | |
[1] Institute of Biochemistry, University of Genoa, Viale Benedetto XV, 1, 16132 Genoa, Italy | |
关键词: Murine erythroleukemia cell; PKC isozyme; Down-regulation; Cell differentiation; PKC; protein kinase C; MEL; murine erythroleukemia; HMBA; hexamethylenebisacetamide; HA; hydroxylhapatite; α1-PKC; PKC isoform detected with anti-α-PKC antibody; showing a lower M r; | |
DOI : 10.1016/0014-5793(94)00359-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In addition to α, δ and ε-protein kinase C, murine erythroleukemia cells contain ζ-PKC and also a c-PKC isoform, named α1, which shows cross-reactivity with an anti-α-PKC antipeptide antibody. In a C44 MEL cell clone, characterized by a high rate of differentiation, both c-PKC forms are expressed at a level higher than that of the N23 MEL cell clone which differentiates at a low rate and contains higher levels of ε-PKC and particularly of the δ-PKC isozyme. In the course of MEL cell differentiation, δ-PKC in N23 cells and α1-PKC in C44 cells are rapidly down-regulated and the overall process is almost completed before cell commitment. Of the other three PKC isozymes present in both clones, only α-PKC is down-regulated to a significant extent. It is proposed that modulation of the signal delivered by each PKC isozyme is one of the biochemical mechanisms involved in MEL cell differentiation.
【 授权许可】
Unknown
【 预 览 】
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