Protein kinase C isoforms in murine erythroleukemia cells and their involvement in the differentiation process

FEBS Letters

Pontremoli, S.¹  Sparatore, B.¹  Passalacqua, M.¹  Melloni, E.¹  Patrone, M.¹  Pessino, A.¹ 

[1] Institute of Biochemistry, University of Genoa, Viale Benedetto XV, 1, 16132 Genoa, Italy

关键词: Murine erythroleukemia cell;    PKC isozyme;    Down-regulation;    Cell differentiation;    PKC;    protein kinase C;    MEL;    murine erythroleukemia;    HMBA;    hexamethylenebisacetamide;    HA;    hydroxylhapatite;    α1-PKC;    PKC isoform detected with anti-α-PKC antibody;    showing a lower M r;   

DOI  :  10.1016/0014-5793(94)00359-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In addition to α, δ and ε-protein kinase C, murine erythroleukemia cells contain ζ-PKC and also a c-PKC isoform, named α₁, which shows cross-reactivity with an anti-α-PKC antipeptide antibody. In a C44 MEL cell clone, characterized by a high rate of differentiation, both c-PKC forms are expressed at a level higher than that of the N23 MEL cell clone which differentiates at a low rate and contains higher levels of ε-PKC and particularly of the δ-PKC isozyme. In the course of MEL cell differentiation, δ-PKC in N23 cells and α₁-PKC in C44 cells are rapidly down-regulated and the overall process is almost completed before cell commitment. Of the other three PKC isozymes present in both clones, only α-PKC is down-regulated to a significant extent. It is proposed that modulation of the signal delivered by each PKC isozyme is one of the biochemical mechanisms involved in MEL cell differentiation.

【 授权许可】

Unknown