Down‐regulation of phorbol diester binding to NG115‐401L neuronal cells is dependent on structure, concentration and time

FEBS Letters

Dreher, Martin L.¹  Hanley, Michael R.¹  Mattingly, Raymond R.¹ 

[1] MRC Molecular Neurobiology Unit, University of Cambridge Medical School, Hills Road, Cambridge CB2 2QH, England

关键词: Phorbol ester;    Protein kinase C;    Phorbol diester binding;    Down-regulation;    (Neuronal cell line);    PDBu;    phorbol 12;    13-dibutyrate;    PMA;    phorbol 12-myristate 13-acetate;    PKC;    protein kinase C;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    BSA;    bovine serum albumin;    HGB;    Hank's saline supplemented with 8 mg/ml glucose and 2 mg/ml BSA;   

DOI  :  10.1016/0014-5793(87)80500-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The down-regulation of [³H]PDBu binding to a neural cell line, NG115-401L, has been examined in response to two biologically active phorbol diesters, PDBu and PMA. Chronic treatment with PDBu or PMA causes a concentration- and time-dependent loss of specific [³H]PDBu binding. The action of PMA is biphasic with respect to both concentration and time dependence.

【 授权许可】

Unknown