Complementary addressed modification of nucleic acids with the alkylating derivatives of oligothymidylate ethyl phosphotriesters Effect of the phosphotriester fragments' configuration

FEBS Letters

Abramova, T.V.¹  Ryte, A.S.¹  Lebedev, A.V.¹  Vlassov, V.V.¹ 

[1] Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: Nucleic acid;    Oligonucleotide derivative;    Affinity modification;   

DOI  :  10.1016/0014-5793(88)80323-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The alkylating derivatives of four individual diastereomers of the oligonucleotide [dTp(Et)]₃dTpU and two individual diastereomers of oligonucleotide [dTp(Et)dTp]₄ have been synthesized. The reagents with the phosphorus atoms in the enantiomeric p″ configuration are shown to be more efficient in reacting with poly(dA) and with nucleic acids in Krebs-2 ascites carcinoma cells compared to those with the phosphorus atoms in the p′ configuration.

【 授权许可】

Unknown