Sequence‐specific chemical modification of a 365‐nucleotide‐long DNA fragment with an alkylating oligonucleotide derivative

FEBS Letters

Gaidamakov, S.A.¹  Gorn, V.V.¹  Grachev, S.A.¹  Vlassov, V.V.¹ 

[1] Institute of Bioorganic Chemistry, Novosibirsk 630090, USSR

关键词: Nucleic acid;    Affinity modification;   

DOI  :  10.1016/0014-5793(85)80345-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An aromatic 2-chloroethylamino group was attached to the 5'-terminal phosphate of the oligodeoxyribonucleotide pCCCTCTTTCTT. The oligonucleotide derivative prepared was used for modification of the 365-nucleotide-long DNA fragment. It was found that modification of the fragment proceeds in a sequence-specific way at 3 guanosine residues within the sequence complementary to the oligonucleotide reagent.

【 授权许可】

Unknown