Complementary addressed modification of oligonucleotide d(pGpGpCpGpGpA) with platinum derivative of oligonucleotide d(pTpCpCpGpCpCpTpTpT)

FEBS Letters

Gorn, V.V.¹  Vlassov, V.V.¹  Ivanova, E.M.¹  Mamaev, S.V.¹  Kazakov, S.A.¹ 

[1] Institute of Organic Chemistry, Siberian Division of the USSR Academy of Sciences, 630090 Novosibirsk, USSR

关键词: Affinity modification;    Platinum reagent;    Cross-linked oligonucleotide;   

DOI  :  10.1016/0014-5793(83)80773-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

New heterobifunctional reagent [BrPt(dien)—(CH₂)₆—(dien)Pt(H₂O)]³⁺ (I) (− dien = diethylenetriamine residue) is proposed for preparation of complementary addressed reagents, reactive derivatives of oligonucleotides and polynucleotides. Two reactive groupings of (I) bind to oligonucleotides at different rates due to the higher reactivity of −[(dien)Pt(H₂O)]²⁺ as compared to −[(dien)Pt Br]⁺. Using (I), bromodiethylenetriaminoplatinum group was attached to the oligonucleotide d(pTpCpCpGpCpCpTpTpT) (II) by rapid reaction of aquatriaminoplatinum group of (I) with guanosine residue of (II). The reactive oligonucleotide derivative III thus obtained was shown to modify predominantly 5′-terminal guanosine residue in the complementary oligonucleotide d(pGpGpCpGpGpA) (IV).

【 授权许可】

Unknown