Fatty acid uptake and metabolism to ketone bodies and triacylglycerol in rat and human hepatocyte cultures is dependent on chain‐length and degree of saturation Effects of carnitine and glucagon

FEBS Letters

Emmison, Neil¹  Agius, Loranne¹ 

[1] Human Metabolism Research Centre, Department of Medicine, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, England

关键词: Fatty acid;    Ketone body;    Triacylglycerol;    Carnitine;    Glucagon;    Cepatocyte (Rat;    Human);   

DOI  :  10.1016/0014-5793(88)80289-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Rat and human hepatocyte cultures were incubated with 5 common plasma longchain fatty acids (C16–C18). Rates of fatty acid uptake were similar in rat and human hepatocytes and were of the order: 16:1 > 16:0; 18:2 > 18:1 > 18:0. Rates of ketogenesis were lower in human compared to rat hepatocytes. In rat hepatocytes glucagon stimulated ketogenesis only in the presence of exogenous carnitine and rates of ketogenesis were higher from unsaturated compared to corresponding saturated fatty acids. Glucagon decreased triacylglycerol secretion irrespective of the fatty acid substrate and it increased intracellular triacylglycerol accumulation. The latter effect of glucagon was more marked in the absence of carnitine supplementation.

【 授权许可】

Unknown