Amino acid sequence of rat liver cathepsin L

FEBS Letters

Towatari, Takae¹  Katunuma, Nobuhiko¹ 

[1] Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan

关键词: Cathepsin L;    Cysteine protease;    Amino acid sequence;    Lysosome;    HPLC;    high-performance liquid chromatography;    SDS-PAGE;    SDS-polyacrylamide electrophoresis;    Con A;    concanavalin A;    MCP;    mouse cysteine proteinase;    TFA;    trifluoroacetic acid;    PTH;    phenylthiohydantoin.;   

DOI  :  10.1016/0014-5793(88)80285-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete amino acid sequences of the heavy and light chains of rat liver cathepsin L (EC 3.4.22.15) were determined at the protein level. The heavy and light chains consisted of 175 and 44 amino acid residues, respectively, and their M _r values without glycosyl groups calculated from these sequences were 18941 and 5056, respectively. The amino acid sequence was also determined from the N-terminal sequences of the heavy and light chains, and the sequences of cleavage fragments of the heavy chain with lysylendopeptidase and cyanogen bromide. The fragments were aligned by comparison with the amino acid sequence deduced from the sequence of cDNA of rat preprocathepsin L. The sequence of rat liver cathepsin L determined at the protein level was identical with that deduced from the cDNA sequence except that in the heavy chain, residues 176–177 (Asp-Ser) were not present at the C-terminus and alanine was replaced by proline at residue 125. Asn-108 in the heavy chain is modified with carbohydrate.

【 授权许可】

Unknown