Meso‐chlorination of chlorophyll a in the course of pigment extraction

FEBS Letters

Kobayashi, Masami²  Scheer, Hugo¹  Struck, Andreas¹  Watanabe, Tadashi² 

[1] Botanisches Institut der Universität, Menzinger Str. 67, D-8000 München 19, FRG;Institute of Industrial Science, University of Tokyo, Roppongi, Minato-ku, Tokyo 106, Japan

关键词: Chlorophyll chlorination;    Pigment extraction;    Photosynthesis;    HPLC analysis;   

DOI  :  10.1016/0014-5793(88)81282-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

HPLC analysis has shown that the meso δ-carbon of chlorophyll (Chl) a undergoes chlorination to give δ-chloro Chl a (Cl-Chl a), possibly by an enzymatic reaction with chloride ion in the wet medium of crushed plant tissue. A sizeable amount, occasionally a 1% fraction, of the whole Chl a is chlorinated during tissue crushing/pigment extraction. The chlorination can be neatly or completely suppressed if plant materials are kneaded with a sufficient amount of a desiccant or with Cl⁻-scavenging silver ion before pigment extraction.

【 授权许可】

Unknown