Synthesis of diadenosine 5′,5‴‐P1,P4‐tetraphosphate by organellar and cytoplasmic phenylalanyl‐tRNA synthetases of Euglena gracilis

FEBS Letters

Krauspe, R.²  Parthier, B.²  Wasternack, C.¹ 

[1] Sektion Biowissenschaften der Martin-Luther-Universität, Halle-Wittenberg, WB Biochemie, Pflanzenbiochemische Abteilung, Neuwerk 1, 4020 Halle/Saale, GDR;Institut für Biochemie der Pflanzen der AdW der DDR, Weinbergweg 3, 4050 Halle/Saale, GDR

关键词: Ap4A synthesis;    Phenylalanyl-tRNA synthetase;    Chloroplast;    Mitochondria;    Ap4A;    diadenosine 5′;    5‴-P1;    P4-tetraphosphate;    PRS;    phenylalanyl-tRNA synthetase;   

DOI  :  10.1016/0014-5793(88)81278-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Purified phenylalanyl-tRNA synthetases present in chloroplasts, mitochondria and cytoplasm of green and bleached Euglena gracilis strains, respectively, are able to synthesize diadenosine 5′,5‴-P¹,P⁴-tetraphosphate (Ap₄A). Ap₄A synthesis is strictly dependent on zinc ions. This is the first evidence that chloroplasts should be able to synthesize Ap₄A. Synthesis of Ap₄A by phenylalanyl-tRNA synthetases of the three compartments of a plant cell or by other enzymes such as Ap₄A phosphorylase is discussed.

【 授权许可】

Unknown