NMR‐invisible lactate in blood plasma

FEBS Letters

Bell, Jimmy D.¹  Sadler, Peter J.¹  Kubal, Gina¹  Brown, Judith C.C.¹ 

[1] Department of Chemistry, Birkbeck College, University of London, Gordon House, 29 Gordon Square, London WC1E 6BT, England

关键词: 1H-NMR;    Lactate;    Blood plasma;    Glycoprotein;    (Human);   

DOI  :  10.1016/0014-5793(88)81238-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Resonances for lactate are broadened in 500 MHz ¹H NMR spectra of human blood plasma and only about one-third is visible in Hahn spin-echo spectra. Similar effects are observed for some other carboxylate anions. Lactate added to the high-M _r fraction of plasma can give rise to peaks which are too broad to observe in either single-pulse or spin-echo spectra. Addition of agents such as NH₄Cl of SDS dramatically increases the intensities of lactate peaks. Some glycoproteins appear to broaden lactate resonances.

【 授权许可】

Unknown