Isolation of protein kinase C subspecies from a preparation of human T lymphocytes

FEBS Letters

Ase, Katsuhiko¹  Shearman, Mark S.¹  Berry, Nicola¹  Kikkawa, Ushio¹  Nishizuka, Yasutomi¹  Oda, Tomiichiro¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: Protein kinase C;    T lymphocyte;    Cell proliferation;    PKC;    protein kinase C;    FPLC;    fast-protein liquid chromatography;   

DOI  :  10.1016/0014-5793(88)80122-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a preparation of purified human T lymphocytes, we were able to resolve a partially purified protein kinase C (PKC) enzyme fraction into two distinct subspecies, of approximately equal activity. Biochemical and immunocytochemical analysis revealed that these fractions closely resembled the type II(β) and type III(α) PKC subspecies previously identified and characterised from brain tissue. These results provide valuable information for further studies on the role of individual PKC subspecies in T lymphocyte proliferation.

【 授权许可】

Unknown