The interaction between the 33 kDa manganese‐stabilising protein and the D1/D2cytochrome b‐559 complex

FEBS Letters

Chapman, David J.¹  Barber, James¹  Gounaris, Kleoniki¹ 

[1] AFRC Photosynthesis Research Group, Department of Pure & Applied Biology, Imperial College of Science & Technology, London SW7 2BB, England

关键词: Reaction center complex;    Manganese-stabilizing protein;    Oxygen evolution;    Affinity chromatography;    Photosystem II;   

DOI  :  10.1016/0014-5793(88)80119-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using affinity chromatography with the extrinsic 33 kDa protein as the immobilised ligand, it was demonstrated that the reaction centre complex of photosystem II, composed of the D₁, D₂ and cytochrome b-559 polypeptides, can directly interact with the 33 kDa protein. By this approach it was possible to purify the reaction centre from solubilised photosystem II core complexes since neither the 47 kDa nor the 43 kDa protein would bind to the ligand.

【 授权许可】

Unknown