Crosslinking between the 33 kDa extrinsic protein and the 47 kDa chlorophyll‐carrying protein of the PS II reaction center core complex

FEBS Letters

Satoh, Kazuhiko²  Enami, Isao¹  Katoh, Sakae² 

[1] Department of Biology, Faculty of Science, Science University of Tokyo, Kagurazaka, Shinjuku-ku, Tokyo 162, Japan;Department of Pure and Applied Sciences, College of Arts and Sciences, University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan

关键词: Crosslinking;    Reaction center complex;    33 kDa protein;    47 kDa protein;    Oxygen evolution;    PS II;    photosystem II;    LHCP;    lightharvesting chlorophyll protein;    DSP;    dithiobis(succinimidylpropionate);    Mes;    2-(N-morpholino)ethanesulfonic acid;    CBB;    Coomassie brilliant blue R-250;   

DOI  :  10.1016/0014-5793(87)80571-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using highly purified oxygen-evolving photosystem II complexes from spinach, the 33 kDa extrinsic protein was found to crosslink with the 47 kDa chlorophyll-carrying protein with a cleavable bifunctional crosslinking reagent, dithiobis(succinimidylpropionate).

【 授权许可】

Unknown