FEBS Letters | |
Flash‐induced electrogenic reactions in the SA(L223) reaction center mutant in Rhodobacter sphaeroides chromatophores | |
Bloch, Dmitry A.3  Semenov, Alexey Yu.3  Cherepanov, Dmitry A.2  Oesterhelt, Dieter1  Bibikov, Sergei I.3  | |
[1] Max-Plank-Institut für Biochemie, München, Germany;Institute of Electrochemistry, Russian Academy of Sciences, Moscow, Russian Federation;A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119899 Moscow, Russian Federation | |
关键词: Bacterial chromatophore; Reaction center complex; Electrogenic reaction; Ubiquinone; Site-specific mutation; Rhodobacter sphaeroides; | |
DOI : 10.1016/0014-5793(94)80230-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The charge transfer events in the SA(L223) reaction center mutant Rhodobacter sphaeroides chromatophores were investigated by direct electrometry. Besides the primary charge separation, the small stigmatellin-sensitive electrogenic reaction due to the electron transfer from the primary to the secondary quinone acceptor in the reaction center complex was observed after the first flash. The second flash-induced electrogenic phase of the secondary quinone protonation and subsequent electrogenic reactions of the cytochrome bc 1 complex were much slower than those in chromatophores of the wild type. It is suggested that replacement of Ser-L223 by Ala impairs both specific proton-conducting pathways leading to the secondary quinone QB.
【 授权许可】
Unknown
【 预 览 】
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RO201912020299281ZK.pdf | 584KB | download |