Isolation of photosystem II reaction center complex by affinity chromatography with the peripheral 33‐kDa polypeptide as ligand

FEBS Letters

Yamamoto, Yasuyuki¹  Yamamoto, Yasusi¹  Nishimura, Mitsuo¹  Isogai, Yasuhiro¹ 

[1] Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812, Japan

关键词: Oxygen evolution;    Photosystem II;    Reaction center;    Affinity chromatography;    33 kDa polypeptide;    Photosynthesis;    Chl;    chlorophyll;    DCIP;    2;    6-dichloro-indophenol;    DPC;    1;    5-diphenylcarbazide;    Mes;    4-morpholineethanesulfonic acid;    Mops;    3-N-morpholinepropanesulfonic acid;    octylglucoside;    n-octyl-β-d-glucopyranoside;    PAGE;    polyacrylamide gel electrophoresis;    PS;    photosystem;   

DOI  :  10.1016/0014-5793(87)80424-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a procedure for one-step isolation of the PS II reaction center complex from PS II particles by affinity chromatography with the peripheral 33-kDa polypeptide as ligand. The CaCl₂-washed PS II particles prepared from spinach chloroplasts were solubilized with n-octyl-β-d-glucopyranoside and subjected to affinity chromatography. The purified complex consisted of polypeptides with apparent molecular masses of 47, 43, 32, 30 and 8 kDa.

【 授权许可】

Unknown