期刊论文详细信息
FEBS Letters | |
Isolation of photosystem II reaction center complex by affinity chromatography with the peripheral 33‐kDa polypeptide as ligand | |
Yamamoto, Yasuyuki1  Yamamoto, Yasusi1  Nishimura, Mitsuo1  Isogai, Yasuhiro1  | |
[1] Department of Biology, Faculty of Science, Kyushu University, Fukuoka 812, Japan | |
关键词: Oxygen evolution; Photosystem II; Reaction center; Affinity chromatography; 33 kDa polypeptide; Photosynthesis; Chl; chlorophyll; DCIP; 2; 6-dichloro-indophenol; DPC; 1; 5-diphenylcarbazide; Mes; 4-morpholineethanesulfonic acid; Mops; 3-N-morpholinepropanesulfonic acid; octylglucoside; n-octyl-β-d-glucopyranoside; PAGE; polyacrylamide gel electrophoresis; PS; photosystem; | |
DOI : 10.1016/0014-5793(87)80424-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report a procedure for one-step isolation of the PS II reaction center complex from PS II particles by affinity chromatography with the peripheral 33-kDa polypeptide as ligand. The CaCl2-washed PS II particles prepared from spinach chloroplasts were solubilized with n-octyl-β-d-glucopyranoside and subjected to affinity chromatography. The purified complex consisted of polypeptides with apparent molecular masses of 47, 43, 32, 30 and 8 kDa.
【 授权许可】
Unknown
【 预 览 】
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RO201912020289902ZK.pdf | 270KB | download |