An increase in intracellular pH is a general response of promonocytic cells to differentiating agents

FEBS Letters

Abita, Jean Pierre³  Frelin, Christian¹  Krawice, Irène³  Ladoux, Annie³  Damais, Chantal² 

[1] Centre de Biochimie du CNRS, Université de Nice, Parc Valrose, 06034 Nice Cedex, France;Laboratoire d'Immunologie, Département des Biotechnologies, Roussel Uclaf, 93230 Romainville, 06034 Nice Cedex, France;INSERM U 204, Hopital Saint Louis, 75475 Paris Cedex 10, France

关键词: intracellular pH;    Na+/H+ exchange;    Interferon;    Phorbol ester;    Vitamin D3;    (Monocyte);    1;    25-(OH)2D3;    1;    25-dihydroxyvitamin D3;    PMA;    12-O-tetradecanoylphorbol-13-acetate;    rIFN;    recombinant γ human interferon;    pHi;    intracellular pH;    EIPA;    ethylisopropylamiloride;   

DOI  :  10.1016/0014-5793(88)80115-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Intracellular pH (pHi) was measured in HL60 and U937 cells before and after differentiation into monocyte-macrophage like cells. 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D₃ all increased pHi. The increases elicited were rapid with PMA, much slower with retinoic acid and interferon and still slower with 1,25-dihydroxyvitamin D₃. Increases in pHi are due to an activation of the Na⁺/H⁺ exchange system. High pHi values are unlikely to serve as an early intracellular signal for initiating monocytic differentiation.

【 授权许可】

Unknown