Na+/H+ exchange is not necessary for protein kinase C‐mediated effects in platelets

FEBS Letters

Wheeler-Jones, Caroline P.D.¹  Krishnamurthi, Sushila¹  Patel, Yatin¹  Morgan, Winston A.¹  Kakkar, Vijay V.¹ 

[1] Thrombosis Research Unit, Rayne Institute, King's College School of Medicine and Dentistry, 123 Coldharbour Lane, London SE5 9NU, England

关键词: Na+/H+ exchange;    Platelet;    Protein kinase C;    [Na+]e;    extracellular Na+;    [pH]i;    intracellular pH;    [Ca2+]i;    intracellular Ca2+;    PMA;    phorbol 12-myristate 13-acetate;    diC8;    1;    2-dioctanoylglycerol;    5HT;    5-hydroxytryptamine;    PKC;    protein kinase C;   

DOI  :  10.1016/0014-5793(89)80908-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The role of Na⁺/H⁺ exchange in protein kinase C-mediated effects in platelets was investigated by studying the effect of removal of extracellular Na⁺ ([Na⁺]_e) on the different responses induced by phorbol 12-myristate 13-acetate (PMA) and 1,2- dioctanoylglycerol (diC₈). None of the responses studied, namely, protein phosphorylation, translocation of enzyme activity to the membrane fraction, potentiatory and inhibitory effects on platelet activation ([Ca²⁺]_i, arachidonate and granule release) showed an absolute dependence on [Na⁺]_e. With the exception of dense-granule release, which was clearly potentiated by the removal of [Na⁺]_e and showed a negative correlation with exchanger activity, the other effects of PMA and diC₈ were not affected by [Na⁺]_e removal. It is concluded that Na⁺/H⁺ exchange is not essential for protein kinase C activation in platelets.

【 授权许可】

Unknown