Demonstration of N‐acetylchondrosine‐degrading β‐glucuronidase in rabbit liver

FEBS Letters

Nakamura, Toshiya¹  Endo, Masahiko¹  Takagaki, Keiichi¹  Majima, Mitsuo¹ 

[1] Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036, Japan

关键词: N-Acetylchondrosine;    β-Glucuronidase;    GlcUA;    D-glucuronic acid;    GlcNAc;    N-acetyl-D-glucosamine;    GalNAc;    N-acetyl-D-galactosamine;    GLC;    gas-liquid chromatography;   

DOI  :  10.1016/0014-5793(88)81310-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

N-Acetylchondrosine was incubated at pH 4.0 with a rabbit-liver crude enzyme extract. Gel filtration of the reaction products on Sephadex G-15 revealed the presence of monosaccharide liberated from the disaccharide. The monosaccharide fraction was analyzed by gas-liquid chromatography, and identified as a mixture of glucuronic acid and N-acetylgalactosamine. These results indicate the presence of β-glucuronidase, which degrades N-acetylchondrosine, in rabbit liver. The discovery of the presence of this enzyme may help to establish the complete degradation process of chondroitin sulfates.

【 授权许可】

Unknown