FEBS Letters | |
Demonstration of N‐acetylchondrosine‐degrading β‐glucuronidase in rabbit liver | |
Nakamura, Toshiya1  Endo, Masahiko1  Takagaki, Keiichi1  Majima, Mitsuo1  | |
[1] Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki 036, Japan | |
关键词: N-Acetylchondrosine; β-Glucuronidase; GlcUA; D-glucuronic acid; GlcNAc; N-acetyl-D-glucosamine; GalNAc; N-acetyl-D-galactosamine; GLC; gas-liquid chromatography; | |
DOI : 10.1016/0014-5793(88)81310-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
N-Acetylchondrosine was incubated at pH 4.0 with a rabbit-liver crude enzyme extract. Gel filtration of the reaction products on Sephadex G-15 revealed the presence of monosaccharide liberated from the disaccharide. The monosaccharide fraction was analyzed by gas-liquid chromatography, and identified as a mixture of glucuronic acid and N-acetylgalactosamine. These results indicate the presence of β-glucuronidase, which degrades N-acetylchondrosine, in rabbit liver. The discovery of the presence of this enzyme may help to establish the complete degradation process of chondroitin sulfates.
【 授权许可】
Unknown
【 预 览 】
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