The amino acid sequence of a trypsin inhibitor from the seeds of Momordica charantia Linn. Cucurbitaceae

FEBS Letters

Qian, Rui-Qing¹  Wang, Yu¹  Zeng, Fu-Yue¹ 

[1] Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai, China

关键词: Amino acid sequence;    Sequence homology;    Trypsin inhibitor;    (Momordica charantia);    MCI;    Momordica charantia inhibitor;    HPLC;    high-performance liquid chromatography;    PITC;    phenylisocyanate;    DABITC;    4-N;    N-dimethylaminoazobenzene-4-isothiocyanate;    DABTH;    4-N;    N-dimethylaminoazobenzene-4-thiohydantoin;   

DOI  :  10.1016/0014-5793(88)81297-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A trypsin inhibitor (MCI-3) was isolated from the seeds of Momordica charantia Linn. Cucurbitaceae in three steps involving the affinity chromatography, CM-Sephadex chromatography and HPLC. It is composed of 62 amino acid residues: Asp₃Thr.₅Ser₄Glu₅Pro₄Gly₇Ala₈Val₉Ile₄Leu₁Lys₃Arg₈Trp₁; contained no cysteine; and its M _r was 7443. The amino acid sequence showed significant homology with ‘potato inhibitor I family’ inhibitors.

【 授权许可】

Unknown